PP242 suppression of AML cell expansion in Ba/F3-ITD/luc/GFP mouse model of leukemia. (A) Peripheral blood obtained from retro-orbital plexus before and 2 hours after PP242 administration. Whole blood was fixed and permeabilized as described in “Flow cytometry.” The cells were then stained with antibodies against pAKT (Ser473), and pS6K (Ser235/236), and the MFI of each intracellular marker in GFP⁺ leukemic cells was assessed using flow cytometry after gating on GFP⁺ cells. (Bi-ii) The leukemia burden was monitored using bioluminescent imaging on days 5 and 14 after leukemia-cell inoculation (i). The bioluminescent intensities were averaged the peak light-emitting exposure in each group of mice and displayed as photons/second in the bar graphs (ii).