Flow cytometry-based discrimination of primary AML cells and MSCs cultured alone or cocultured. Primary AML cells (A) or AML MSCs (B) cultured alone and used as controls; or harvested from AML/MSC coculture (C) were fixed, permeabilized, and stained with the indicated cell surface markers. (C) Contour blot depicting different population of attached AML cells and MSCs (left) and floating AML cells (right) detected by flow cytometry in the coculture setting. Blue, CD90(−) AML cells; green CD34⁺ AML progenitor cells; red, CD90⁺ AML MSCs.