Characterization of disheveled-associated activator of morphogenesis 1 in platelets. (A) DAAM1 quantitative RT-PCR was completed using ET or normal platelets (N = 5/group). (B) Normalized DAAM1-specific spectral counts from MudPit analyses are expressed as the mean ± SEM from 3 normal and 3 ET subjects. (C) DAAM1 peptide abundance was quantified between cohorts for each of 2 peptides and expressed as the mean ± SEM (N = 3/cohort). (D) Immunoblot analysis was completed using 4%-15% SDS-PAGE for each of 6 platelet lysates. (E) Quantitative multiple reaction monitoring from one normal and one ET platelet sample is displayed as the area under the curve (in parentheses) of the m/z 1215.8 transition to its doubly charged Y₁₉ ion. (F) MS/MS spectra of the DAAM1 peptide IQPDEFFGIFDQFLQAVSEAK specifies the fragmentation and Y₁₉ ion(s) used for abundance quantification.