Cellular and cohort-specific miRNA expression. (A) Quantitative RT-PCR was used to quantify miRNA expression patterns in leukocytes (WBC), normal platelets, or thrombocytosis (ET or RT), displayed by group mean (N = 5 samples/group). The threshold sensitivity of miR 490 3p and 5p assays is delineated by boundaries of the stippled box. (B-C) Normalized aggregate expression data for miR 490 3p and miR 490 5p are displayed for microarray (B; N = 79) or quantitative RT-PCR data (C; N = 10/group). Data are presented as the mean ± SEM.