DEP-1, via Y1311 and Y1320, mediates the formation of branching capillary-like structures. (A) HUVECs transfected with control (CTL) or DEP-1 siRNAs were plated 48 hours after transfection on Matrigel (in duplicates) to promote the formation of capillary-like structures. The length of tubes formed as well as the number of branches at connecting nodes were quantified after 5-6 hours from 6 fields at ×10 magnification /well. Results ± SD have been normalized using the average from the control condition and are representative of 3 independent experiments; *P < .05. (B) HUVECS transfected with the indicated constructs were plated in duplicates on Matrigel to evaluate their ability to form branching capillary-like structures. Quantification was done as described in panel A and is representative of 3 independent experiments; *P < .05. (C) Representative DEP-1 expression level in RNAi-transfected cells. (D) Comparable expression level of DEP-1 and mutants in transfected HUVECs.