VPS33B binds to C14orf133 in HEK 293 cells, Dami cells, and megakaryocytes. (A) Myc-tagged C14orf133 (Myc-C14orf133) was coimmunoprecipitated (IP) with GFP-tagged VPS33B (GFP-VPS33B) or VPS33A (GFP-VPS33A) after cotransfecting HEK293 cells. The IP antibody was anti-Myc whereas a nonspecific mouse IgG (mIgG) was used as a control. The immunoblot (IB) signals were detected using an anti-GFP antibody. Myc-C14orf133 coimmunoprecipitated with GFP-VPS33B but not GFP-VPS33A. (B) Using anti-VPS33B serum and preimmune serum as control, endogenous VPS33B was immunoprecipitated from Dami and human megakaryocyte cells. The immunoblot signals were detected with anti-C14orf133 mouse serum. VPS33B binds to C14orf133 endogenously in Dami and megakaryocyte cells.