American Society of Hematology

Figure 2

$Figure 2. Lineage-specific hematopoietic defects in the BM of Rs1 mice. Twelve-week-old (12 wk) control (Ctrl) and ColI(2.3)+/Rs1+ (Rs1) mice (n = 8-13 per group) were used for these analyses. (A) Schematic overview and (B) gating strategy for the different hematopoietic lineages investigated (gray represents populations decreased in frequency in Rs1 mice). CLP indicates common lymphoid progenitor; B, B-cell; CMP, common myeloid progenitor; GMP, granulocyte/macrophage progenitor; MEP, megakaryocyte/erythrocyte progenitor; E, erythrocyte; Plt, platelet; Gr, granulocyte; and M, macrophage. The myeloid progenitor (MP) population containing CMPs, GMPs, and MEPs and the LSK population containing HSCs and MPPs are boxed. (C) Frequencies of the indicated populations in the BM. (D) Methylcellulose read-out for myeloid CFU activity in 12 500 unfractionated Ctrl and Rs1 BM cells. (E) Schematic overview and (F) gating strategy for detailed analyses of megakaryopoiesis and erythropoiesis (gray represents populations decreased in frequency in Rs1 mice). Pre-MegE indicates megakaryocyte/erythrocyte precursor; MkP, megakaryocyte precursor; Mk, megakaryocyte; CFU-E, colony-forming unit-erythrocyte; I, pro-erythroblast; II, basophilic erythroblast; III, late basophilic/polychromatophilic erythroblast; and IV, orthochromatic erythroblast. (G) Frequencies of the indicated populations in the BM. Data are mean ± SD. *P ≤ .05. **P ≤ .01. ***P ≤ .001.$

Lineage-specific hematopoietic defects in the BM of Rs1 mice. Twelve-week-old (12 wk) control (Ctrl) and ColI(2.3)⁺/Rs1⁺ (Rs1) mice (n = 8-13 per group) were used for these analyses. (A) Schematic overview and (B) gating strategy for the different hematopoietic lineages investigated (gray represents populations decreased in frequency in Rs1 mice). CLP indicates common lymphoid progenitor; B, B-cell; CMP, common myeloid progenitor; GMP, granulocyte/macrophage progenitor; MEP, megakaryocyte/erythrocyte progenitor; E, erythrocyte; Plt, platelet; Gr, granulocyte; and M, macrophage. The myeloid progenitor (MP) population containing CMPs, GMPs, and MEPs and the LSK population containing HSCs and MPPs are boxed. (C) Frequencies of the indicated populations in the BM. (D) Methylcellulose read-out for myeloid CFU activity in 12 500 unfractionated Ctrl and Rs1 BM cells. (E) Schematic overview and (F) gating strategy for detailed analyses of megakaryopoiesis and erythropoiesis (gray represents populations decreased in frequency in Rs1 mice). Pre-MegE indicates megakaryocyte/erythrocyte precursor; MkP, megakaryocyte precursor; Mk, megakaryocyte; CFU-E, colony-forming unit-erythrocyte; I, pro-erythroblast; II, basophilic erythroblast; III, late basophilic/polychromatophilic erythroblast; and IV, orthochromatic erythroblast. (G) Frequencies of the indicated populations in the BM. Data are mean ± SD. *P ≤ .05. **P ≤ .01. ***P ≤ .001.

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