Metformin administration enhances hematopoiesis. (A) CBCs after 6 months of treatment with metformin. D2, Fancd2; HGB, hemoglobin; NS, not significant. The data are pooled results from 17 to 19 individual mice in each group. (B) Representative flow cytometry profiles for placebo and metformin-treated Fancd2^−/− mice. The percentages on the profiles indicate the mean value for each group. PI, propidium iodide. (C) Quantification of CD34⁻KSL frequency in bone marrow. The data represent the percentage of CD34⁻KSL cells in all nucleated bone marrow cells from 15 mice in each group.