CD146 is required in VEGF-induced cell migration and tube formation. (A) Cell migration was determined using a Transwell system. HUVECs were induced by VEGF in the presence of anti-CD146 mAbs AA98 or AA1. The migrating cells were counted. Data were collected from 3 wells. Representative images of migrated cells are also shown. (B) VEGF-induced tube formation was quantified in the presence of anti-CD146 mAb AA98 or AA1 by counting the total vessel length per field. Data were collected from 3 wells. (C) Cells cotransfected with CD146-siRNA and pcDNA3.1-CD146 were subjected to cell-migration assays and the number of cells migrating through the filters was recorded. The number of cells per optic field is presented as the mean ± SEM of at least 3 independent assays. (D) Cells cotransfected with CD146-siRNA and pcDNA3.1-CD146 were subjected to tube-formation assays. Results presented are total tube lengths from 3 independent tests (means ± SEM). *P < .05; **P < .01; ***P < .001. NS indicates not significant.