VEGFR-2 signaling is dependent on CD146 binding to Moesin. (A) A CD146-▵KKGK was transfected transiently into HUVECs and activation of VEGFR-2 induced by VEGF and signaling to AKT and p38 was determined. (B) Moesin-▵ABD was transfected transiently into HUVECs and activation of VEGFR-2 on induction with VEGF and signaling to AKT or p38 were determined. (C-D) The migrating capability of HUVECs induced by VEGF was determined after transfection with CD146-▵KKGK or Moesin-▵ABD. Cell migration was determined using a Transwell system as described in “Cell migration assay.” Data were collected from 3 wells. Representative images of migrated cells are also shown. *P < .05; **P < .01. NS indicates not significant.