miR-27a/b regulate embryonic vessel formation in zebrafish. (A-C) miR-27a and miR-27b morpholinos were injected into zebrafish at 1-cell or 2-cell stage. The 48-hpf vessel phenotype was quantified for ISV and DLAV (A), and PAV (B). n = 112 to 152. (A) Data are number of defects per animal. (B) Quantification of PAV phenotype: 0 indicates absent; 1, partially absent; and 2, present. (C) Representative images of panels A and B. Vasculature of tg(fli1:EGFP) zebrafish embryos were stained by antibody against GFP (48 hpf). Black arrows indicate ISV or DLAV defects. *PAV defects. (D-E) miR-27a and miR-27b morpholinos were injected into zebrafish, and intracranial bleeding was monitored at 48 hpf and at 72 hpf. (D) Quantification of intracranial bleedings (n = 26-90). (E) Representative images of intracranial bleedings (72 hpf). Black arrows indicate hemorrhages.