Effect of miR-27a/b inhibition on endothelial cell sprouting. HUVECs were transfected with miR inhibitors as indicated. (A-B) Expression of mature miR-27a (A) and miR-27b (B) was detected by quantitative RT-PCR compared with control-precursor (pre-miR-Co) transfected control cells after 24 hours. Data were normalized to RNU48. *P < .05 (paired t test). n = 3 to 8. (C) Effect of miR-27a/b inhibition on endothelial cell sprout formation in the spheroid assay (n = 10 spheroids/experiment, n = 1 for 0.01nM, and n = 3-8 experiments for 0.1-100nM). *P < .05 versus respective control inhibitors. (D) Effect of miR-27a/b inhibition on endothelial cell viability (MTT assay; n = 3).