Effect of miR-27a/b overexpression on endothelial cell sprouting. (A-B) HUVECs were transfected with miR-27a precursor (pre-miR-27a, A) and miR-27b precursor (pre-miR-27b, B) as indicated. Expression of mature miR-27a/b was detected by quantitative RT-PCR compared with control-precursor (pre-miR-Co) transfected control cells after 24 hours. Data were normalized to RNU48; n = 3 to 7. *P < .05 versus pre-Co. (C-D) Effect of pre-miR-27a/b on endothelial cell sprout formation in the spheroid assay (n = 10 spheroids/experiment; n = 5-13 experiments). *P < .05 versus pre-miR-Co. Representative images of spheroids are shown in panel D.