Absence of LTB4/BLT1 axis facilitates induction of diverse memory CD4⁺ T subsets and polarized Th2/Th17 cells with antitumor phenotype in KO/WGM mice in late phase. TDLNs cells harvested from WT/WGM or WT/WGM mice (n = 3-5/group) on day 46 after the FTC were subjected to polychromatic flow cytometric analyses. (A) Dot plot profiles (top panels) and graphs (bottom panels) represent the percentages of CD4⁺CD44⁺CD62L⁺ T_CM or CD4⁺CD44⁺CD62L⁻ T_EM subset relative to the total CD4⁺ T-cell population. Numbers in boldface in dot plot profiles show the representative percentages of T_CM and T_EM in WT/WGM and KO/WGM and are reflected to the graphs. (B) Bar graphs represent the total number of IL-4-producing T cells in TDLNs derived from WT/WGM and KO/WGM mice (n = 3 or 4). Combined data from 2 independent experiments are shown. (C) Th17 adoptive T-cell transfer (ACT) assay. Splenic CD4⁺ T cells from WT/WGM or KO/WGM mice on day 46 were MACS-sorted and stimulated with plate-bound anti-CD3 mAb and soluble anti-CD28 mAb under Th17 condition. After 4 days of incubation, 1 million cells were intravenously transferred into recipient syngeneic BALB/c mice. On the next day, they received subcutaneous challenge with WEHI3B cells in the right flank. Bar graph represents the tumor volume (mm³) of untreated, mice treated with WT/WGM mice- or KO/WGM mice-derived Th17 ACT, assessed on day 10 after the Th17 ACT therapy. (D-E) Phenotypic profile of immune-regulatory T cells in late phase. Shown are representative dot plots depicting the percentages of (D) CD3⁺CD4⁺CD25⁺FoxP3⁺ cells in TDLNs harvested from WT/WGM or KO/WGM mice, and of (E) CD3⁺CD4⁺GITR⁺ T cells in TDLNs and spleen from WT/WGM or KO/WGM mice. Bar graphs represent the mean ± SEM. Significant differences: *P < .05, **P < .01. Representative data from 3 independent experiments with similar results or combined data (A-C) from 2 independent experiments are shown.