DR3-activated Foxp3⁺Tregs show a distinct gene expression profile. (A) The gating strategy for Treg isolation from FoxP3.Luci.DTR-4 mice. (B) Gene expression profiles of Iso-Treg and DR3-Treg. The expression changes in chemokine/cytokine, transcription factor, cell cycle/turn over and effector/activation molecules are shown as a heat map, where red and green represent the highest- and lowest-expressed genes, respectively.