Induction of miR-378-3p is specific to IL-4–mediated signaling in MΦ. (A) Thio-elicited, adherence-purified MΦ were incubated with rIL-4 (black squares), rM-CSF (gray circles), or with medium alone (open squares) for the indicated time and analyzed for miRNA and mRNA expression. Statistics indicate differences between rM-CSF–treated samples and rIL-4 controls. ***P < .001. Each data point represents mean and SEM of 6 individual animals. Results from a single experiment shown. (B) CD11b⁺F4/80⁺ (MΦ), CD19⁺F4/80⁻ (B cells), CD4⁺CD11b⁻ lymphocytes (CD4⁺) and CD8⁺CD11b⁻ lymphocytes (CD8⁺) were FACS-sorted from IL-4c injected (filled bars) or PBS-injected control animals (open bars) and subjected to qRT-PCR. Data are depicted as fold change above PBS controls. Statistics indicate differences between IL-4c–treated samples and PBS-controls. ***P < .001; *P < .05. Bars depict mean and SEM of 10 individual animals per group pooled from 2 independent experiments, except for the CD8⁺ data, which is from 1 representative experiment. The second experiment is not shown because Ccna2 in the PBS controls was not detectable resulting in a fold change of infinity for the IL-4c treatment.