Akt inhibition and miR-378-3p overexpression negatively regulate MΦ proliferation. (A) BALB/c mice were injected intraperitoneally with a single dose of triciribine (1 mg/kg) or vehicle control 1 hour before injection of IL-4c or PBS. After 24 hours cells were analyzed for proliferation and alternative activation by FACS analysis. Each data point is representative of an individual animal. Pooled data from 3 separate experiments shown. (B) Conversion of alamarBlue by RAW264.7 cells 24 and 48 hours after transfection with a miR-378-3p mimic (black squares) or inhibitor (black circles) or the appropriate negative controls (open symbols). Data representative of 3 separate experiments. (C) FACS-analysis of Vybrant CFDA SE dilution in the cells analyzed in panel A. (D) Gene expression of miR-378-3p target genes and genes associated with cell proliferation in RAW264.7 cells 48 hours after transfection with a miR-378-3p mimic (black bars) or the appropriate negative control (open bars). ***P < .001; **P < .01; *P < .05; n.s. indicates not significant.