Akt-inhibition modulates alternative activation of MΦ in vitro. Thio-MΦ were stimulated with rIL-4 (+) or medium (−) after preincubation with the indicated concentration of triciribine for 1 hour and analyzed for expression of alternative activation markers by qRT-PCR. Data are representative of 3-4 animals per group. Error bars indicate SEM. One of 4 experiments shown. Asterisks indicate statistical differences compared with IL-4–treated samples. ***P < .001, **P < .01.