Validation of differential expression of miRNAs in AAMΦ in vivo and in vitro. (A) Peritoneal MΦ were isolated from Thio-injected BALB/c mice or from B malayi–infected BALB/c (WT) or IL-4Rα^−/− (−/−) mice and expression of the indicated miRNAs assessed by qRT-PCR. Each data point shown reflects data from individual mice. (B) Chi3l3 expression in the cells isolated in panel A. (C) Thio-elicited, adherence-purified MΦ were incubated with rIL-4 (IL-4), LPS/IFNγ (L/I), or without stimulus (−) for 16 hours and analyzed as in panel A. (D) Chi3l3 expression in the cells isolated in panel C. One of 3 separate experiments shown. *P < .05; **P < .01; ***P < .001; n.s. indicates not significant.