Clinicopathologic findings in peripheral T-cell lymphomas with TP63 rearrangements. (A) Dual-fusion (D-) and/or breakapart FISH probes were used to screen 190 PTCLs for TP63 rearrangements. In this case, D-FISH showed 2 abnormal fusion signals (arrows), corresponding to fusion of TBL1XR1 (green) to TP63 (red). The remaining green and red signals represent the nonrearranged copies of TBL1XR1 and TP63, respectively. (B) Most PTCLs with TP63 rearrangements showed a diffuse, sheet-like growth pattern (hematoxylin and eosin, 100× magnification; image acquired using an Olympus DP71 camera and Olympus BX51 microscope). (C) All rearranged cases demonstrated apoptotic debris, and 4 of 11 cases had prominent tingible body macrophages (TBM) phagocytozing cellular debris (1000×). Mitotic figures (MF) also were present. TC indicates tumor cells. (D) Immunohistochemistry of the tumor shown in panels A through C shows strong, uniform nuclear staining for p63 protein (400×). Virtually all tumor cells express the proliferation marker Ki67, as well as the lymphocyte activation marker, CD30. Top right panel indicates isotype control antibody. (E) Associations between TP63 rearrangement and results of immunohistochemical studies. (F) PTCL patients with TP63 rearrangements (n = 11) had significantly poorer overall survival than those without TP63 rearrangements (n = 179; median survival with and without rearrangements, 17.9 months vs 33.4 months, respectively).