Up-regulation of PD-1 and Tim-3 is induced by TLI and ATS conditioning and declines after transplantation of bone marrow. (A-C) Percentages of PD-1^hi cells among gated CD8⁺ (A), CD4⁺CD25⁻ (B), or CD4⁺CD25⁺ (C) T cells from the spleen are shown in untreated WT BALB/c mice (UNT WT, n = 8), ATS conditioned WT BALB/c mice (ATS, n = 7), TLI conditioned WT BALB/c mice (TLI, n = 7), ATS + TLI conditioned WT BALB/c mice (ATS + TLI, n = 8), and ATS + TLI conditioned WT BALB/c mice given transplants (WT TX, n = 11). (D) Mean percentages (± SEM) of PD1^hi cells among CD4⁺CD25⁺ T cells (filled square; n = 10), or CD4⁺CD25⁻ T cells (filled circle; n = 10) in PBMCs at different time points after transplantation in WT BALB/c hosts. (E) Mean percentages (± SEM) of PD1^hi among CD8⁺ T cells (filled circle; n = 10), or PD1^hiTim-3⁺ (filled triangle; n = 10) among gated CD8⁺ T cells in PBMCs at different time points after transplantation in WT BALB/c hosts.