ROCK suppression-induced ECs express EC-specific genes. (A) Diagram of cell preparations for phenotype analyses of EC and MC differentiation. Flk1⁺ MPCs were incubated with only VEGF-A (50 ng/mL; Control) and VEGF-A (50 ng/mL) plus Y27632 (10μM; Y27632) for 2 days. (B) Representative FACS analysis for phenotypic markers in CD144⁺ cells; pan-endothelial, mural, and arterial, venous, and lymphatic ECs. (C) Diagram of cell preparations for analyses of gene expression related to differentiation of ECs and MCs. The cells in diagram panel A were sorted with anti-ICAM2 antibody, and gene expressions in each population of ICAM2⁺ ECs were analyzed by quantitative RT-PCR. (D) Comparisons of EC and MC differentiation-related gene expressions. Each group, n = 3. *P < .01 versus each Control.