American Society of Hematology

Figure 3.

$Figure 3. ROS is a major cause of Dox-triggered cell death in ABC-DLBCL cell lines. (A) Changes in cellular ROS levels and viability during a 40-hour Dox treatment. Data from 2 representative ABC-DLBCL cell lines, Ly3 and Ly10, are shown. Viable fraction is defined as Annexin V-neg/Propidium Iodide (PI)-neg cells. (B) The effect of antioxidants on Dox-induced ROS accumulation and cell viability change. Ly3, Ly10, and Riva cells were pretreated with either N-acetyl-cysteine or vitamin C for 1 h before exposing to Dox for 18 hours. ROS measurement was based on mean fluorescent intensity of MitoSox staining, followed by flow cytometry. For Ly3 and Ly10, cell death was defined as the proportion of all Annexin V+ cells. For Riva, viable cells were enumerated based on Trypan blue exclusion. Results shown are mean ± SD and are representative of 2 independent experiments. Two-tailed Student t test was used for pairwise comparison, as indicated. *P < .05; **P < .01; ns, not significant. nil, vehicle control.$

ROS is a major cause of Dox-triggered cell death in ABC-DLBCL cell lines. (A) Changes in cellular ROS levels and viability during a 40-hour Dox treatment. Data from 2 representative ABC-DLBCL cell lines, Ly3 and Ly10, are shown. Viable fraction is defined as Annexin V-neg/Propidium Iodide (PI)-neg cells. (B) The effect of antioxidants on Dox-induced ROS accumulation and cell viability change. Ly3, Ly10, and Riva cells were pretreated with either N-acetyl-cysteine or vitamin C for 1 h before exposing to Dox for 18 hours. ROS measurement was based on mean fluorescent intensity of MitoSox staining, followed by flow cytometry. For Ly3 and Ly10, cell death was defined as the proportion of all Annexin V+ cells. For Riva, viable cells were enumerated based on Trypan blue exclusion. Results shown are mean ± SD and are representative of 2 independent experiments. Two-tailed Student t test was used for pairwise comparison, as indicated. *P < .05; **P < .01; ns, not significant. nil, vehicle control.

This Feature Is Available To Subscribers Only