Central neurons and blood vessels are anatomically coupled in the retina. (A) Three-dimensional reconstruction of an adult mouse retinal flatmount. The image depicts the inner retinal vascular plexus (red; stained with isolectin B4) interwoven with retinal ganglion neurons and their axons (green; stained with β-III tubulin). (B) Corrosion cast of mouse retinal vasculature imaged by environmental scanning electron microscopy. The image shows a network of microcapillaries, veins, and arteries and illustrates the complex wiring of retinal vascular beds. Scale bar: (A) 400 μm; (B) 50 μm.