Effects of SGI-1776 treatment on Pim kinases targets. Effect of SGI-1776 on phospho–c-Myc (Ser62) and total c-Myc (A) phospho-Histone H3 (Ser10) and total Histone H3 (B), phospho-Bad (Ser112) and total Bad (C), and phospho–4E-BP1(Thr37/46) and total 4E-BP1 (D) protein expressions. JeKo-1 and Mino cell were treated with 0.1, 1, 3, 5, or 10μM SGI-1776 for 24 hours, and then cell lysates were analyzed via immunoblot. Quantitation of immunoblots with phosphorylation targets (c-Myc, Histone H3, Bad, and 4E-BP1) is shown in panel E for JeKo-1 cells and in panel F for Mino cells. Phospho-to-total protein ratios were calculated, and data are presented as means of 3 independent experiments ± SEM.