MiR-125b expression decreases in CLL patients and in CLL-derived cell lines. The relative levels of miR-125b (A) and miR-155 (B) in patients with CLL were determined by qRT-PCR. P values (Student t test) are given for indolent (n = 35) and aggressive (n = 38) versus healthy (n = 15; A, left) and for indolent versus aggressive (A, right). Boxes include values from the first to the third quartiles; o indicates extreme data points; +, median; x, mean ± SD. The relative levels of miR-125b (C) and miR-155 (D) in CLL cell lines (MEC1 and MEC2), a B-lymphoblastoid leukemia cell line (CCRF-SB), Burkitt lymphoma DAUDI B lymphoblasts, T cells (CCRF-CEM, Jurkat, and K812F), and monocytes (THP-1 and U937) were determined by qRT-PCR. qRT-PCRs were run in triplicate with the use of 3 different cDNAs (C-D).