IFNγ secreted by CD16-activated Vδ2^neg γδ T cells inhibits HCMV multiplication. The long-term CD16^pos Vδ2^neg γδ T-cell line was incubated with a control mAb or the agonist anti-CD16 mAb for 24 hours with or without IL12. Then, the supernatants were harvested and added on HCMV-infected FSF. As controls, zero (medium alone) or 10 UI/mL of recombinant IFNγ were added on HCMV-infected FSF. Blocking anti-IFNγ mAb was added when indicated. After 4 days in culture, HCMV replication in the supernatants of FSF was quantified using a real-time quantitative HCMV PCR assay. The experiment was performed in duplicate (mean ± SD) and is representative of 2 different experiments.