American Society of Hematology

Figure 5

$Figure 5. Hsp70 does not colocalize with active caspase-3 or GATA-1 in the nucleus of MDS erythroblasts. Erythroblasts from MDS (n = 7) and controls (n = 4) harvested at day 14. (A) Immunolocalization of Hsp70 and active caspase-3. DAPI for nuclei. Merge represents both active caspase-3 (red) and Hsp70 (green). (B) Immunolocalization of GATA-1 and Hsp70 in polychromatophilic erythroblasts. GATA-1 (red), Hsp70 (green), and DAPI (blue). Merge represents the superimposition of all 3 colors. (C) Expression of GATA-1 and Hsp70 in MDS erythroblast nuclei. MDS or control cells are harvested at day 7, 10, and 14. Immunoblot for GATA-1 or Hsp70 in the nuclear fraction. Hsc70 as loading control. Representative experiments are shown.$

Hsp70 does not colocalize with active caspase-3 or GATA-1 in the nucleus of MDS erythroblasts. Erythroblasts from MDS (n = 7) and controls (n = 4) harvested at day 14. (A) Immunolocalization of Hsp70 and active caspase-3. DAPI for nuclei. Merge represents both active caspase-3 (red) and Hsp70 (green). (B) Immunolocalization of GATA-1 and Hsp70 in polychromatophilic erythroblasts. GATA-1 (red), Hsp70 (green), and DAPI (blue). Merge represents the superimposition of all 3 colors. (C) Expression of GATA-1 and Hsp70 in MDS erythroblast nuclei. MDS or control cells are harvested at day 7, 10, and 14. Immunoblot for GATA-1 or Hsp70 in the nuclear fraction. Hsc70 as loading control. Representative experiments are shown.

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