In vivo imaging of platelets, neutrophils, and monocytes during thrombus formation. (A-B) Representative images of (A) Ly-6G or (B) platelets and Ly-6G neutrophils at site of injury in wild-type mice (26 thrombi, 3 mice). (C) Thrombus formation in mice expressing CX3CR1^GFP after infusion of CD41 and Ly-6G antibody. Monocytes (green), platelets (red), and neutrophils (blue) are visualized over time after laser injury. (D) Representative images showing an inflammatory monocyte rolling on a thrombus. The monocyte is depicted in white (composite of blue plus green plus red) or in light blue (composite of dark blue and green). Platelets are in red, neutrophils are in dark blue, monocytes, macrophages and DC are depicted in green. (E) Platelets and fibrin generation in wild-type mice and FXII^−/− mice. Graphs represent medians of fibrin-integrated fluorescence intensity in wild-type mice (29 thrombi, 3 mice) and FXII^−/− mice (30 thrombi, 3 mice) over time.