Real-time imaging of leukocyte accumulation at the site of injury in wild-type mice. (A) Representative images of cells (white arrows) accumulating at the site of injury (n = 32; 3 mice). (B) Exogenously-labeled leukocytes (blue) were detected at the site of injury (top panel). (C) Accumulation of Mac-1 (blue), but not the irrelevant antibody, was detected at the site of thrombus formation (white arrows; middle panel). Images are representative of 32 thrombi performed in 3 mice for each condition. (D) Detection of leukocytes before (top panel) or after (bottom panel) depletion of platelets. The graphs depict the median of the maximal integrated fluorescence intensity of CD41 (left panel) and Mac-1 (right panel) corresponding to platelets and leukocytes accumulation, respectively (31 thrombi; 3 mice); *P < .05.