Capture and migration of mouse neutrophils from whole blood for TNF-Tg and WT mice. (A-C) Chemoattractant (fMLP) dose-response for neutrophils obtained from WT mice from a tail vein puncture, showing the chemotactic index, speed, and directional velocity (n = 3). (D-E) Representative micrographs of neutrophil capture out of whole blood for arthritic (TNF-Tg) and WT mice. (F-I) Difference in the number of neutrophils adhering to the P-selectin substrate, chemotactic index, directional velocity, and speed for neutrophils from TNF-Tg and WT mice. **P < .01. *P < .05. (F) n = 5. (G-I) n = 3. No observable migration for control (no fMLP). (D-E) Scale bars represent 100 μm. Error bars represent SEM. Phase-contrast images acquired using Slidebook Version 5.0.0.14 software with an Olympus IX-81 microscope using a 10× (NA = 0.30) objective at 37°C. Additional details on imaging are given in “Microscopy.”