NF-Ya is expressed in all hematopoietic tissues and lineages and can be deleted efficiently. (A) RNA from BM cells, splenocytes, thymocytes (thy), and cells from the lymph nodes (LNs) of wt mice was analyzed for NF-Ya expression by RT-PCR. (B) Total BM cells, splenocytes, and thymocytes were isolated from wt mice and different blood cell lineages purified with the indicated Abs and magnetic beads. Cell lysate from these cells was subjected to Western blot analysis for NF-Ya (top) and actin (as a loading control; bottom). A black vertical line has been inserted to indicate a repositioned gel lane. (C) RNA from total BM of control and ko mice 1 day and 3 days after a single pIpC injection was subjected to quantitative RT-PCR for NF-Ya. GAPDH was used as an internal control; results were normalized to control samples (n = 6); **P < .01. (D) Protein from BM cells of control, het, and ko mice 4 days after the first pIpC injection was subjected to Western blot analysis with the use of Abs against NF-Ya (left) and actin (as loading control; right). (E) RNA from total BM of control and ko mice 1 day after a single pIpC injection was subjected to quantitative RT-PCR for the indicated genes. GAPDH was used as an internal control; the results were normalized to control samples (n = 6); *P < .05 and **P < .01. (F) RNA from HSCs (LSK/SLAM) of control and ko mice 1 day after a single pIpC injection was subjected to quantitative RT-PCR for the indicated genes. GAPDH was used as an internal control; the results were normalized to the control samples (n = 6); **P < .01.