Effect of A11 on the viability of tumor cells and endothelial cells in vitro and in vivo. (A-C) Effect of A11 on the viability of (A) B16, (B) LLC, and (C) HUVECs as evaluated by the MTT assay. Data are mean ± SD of 3 different determinations. (D) Effect of A11 on the chemotaxis of tumor cells and HUVECs. Chemotaxis was performed in Transwell plates as described in “Transwell migration assay.” Data and SD are given for 3 independent experiment results in which each concentration represent 4 measurements. (E) Effect of A11 on the s.c. tumor growth. Tumor dimensions were measured with calipers for calculation of tumor volume. n = 5; error bars indicate SD.