Ag routing to MHC class-I pathway in IFN-DCs. CLSM examinations of DCs that were allowed to internalize soluble OVA-FITC (A) or NS3 (B) proteins (green) for 15 minutes at 37°C and then were washed and stained for intracellular MHC-I (red) detection. In panel B, cells were double stained with anti-NS3 Ab to visualize the internalized Ag. Insets represent separate channel images. Colocalization was detected in yellow. Representative examples of 5 independent experiments are shown.