Functional studies of CD86 and HLA-G–positive T cells. (A) Flow sorted HLA-G–negative T cells were labeled with CFSE and cocultured with and without HLA-G–positive T cells. After 4 days of culture, cell proliferation was assessed and the inhibition of proliferation by the HLA-G–positive T cells was determined. (B) The inhibition of HLA-G⁺ T cells compared with inhibition by CD86⁺ T cells and CD4⁺CD25⁺⁺CD127⁻ Treg cells in cocultures