Elevated hematocrits and RBCs in ROCK1-deficient mice in response to stress. WT and ROCK1^−/− mice were treated with PHZ (90 mg/kg at day 0). At the indicated time points (3 and 5 days), peripheral blood (PB; 20 μL/mouse) was collected from WT and ROCK1^−/− mice. RBC (M/μL), Hb (g/dL), HCT (%), MCV (fL), MCH (pg), (MCHC g/dL), and RDW (%) in each sample was counted using an automated hemovet. Mean ± SEM of blood counts are presented in the line chart. (A) HCT, (B) Hb, and (C) RBC. For each analysis, (n = 6 mice per genotype for the 3 day time point; n = 5-7 mice per genotype for the 5 day time point and 3 mice per genotype for PBS condition).