Figure 4
RE;c-KitD814V and RE;c-KitT417IΔ418-419 AML phenotypes are clonal. Southern blot analysis using genomic DNA isolated from whole splenocytes obtained from 5 independent, moribund primary RE;c-KitD814V or RE;c-KitT417IΔ418-419 mice. Lane numbers represent the same DNA sample used for both blots. Blots were hybridized with radiolabeled probes complementary to ETO or c-Kit sequences to detect unique retroviral integrants. Arrows indicate the endogenous murine Eto and c-Kit bands. Wild-type C57BL/6 (B6) splenocytes served as control samples for each blot. Asterisks indicate unique retroviral integrants in each sample.

RE;c-KitD814V and RE;c-KitT417IΔ418-419 AML phenotypes are clonal. Southern blot analysis using genomic DNA isolated from whole splenocytes obtained from 5 independent, moribund primary RE;c-KitD814V or RE;c-KitT417IΔ418-419 mice. Lane numbers represent the same DNA sample used for both blots. Blots were hybridized with radiolabeled probes complementary to ETO or c-Kit sequences to detect unique retroviral integrants. Arrows indicate the endogenous murine Eto and c-Kit bands. Wild-type C57BL/6 (B6) splenocytes served as control samples for each blot. Asterisks indicate unique retroviral integrants in each sample.

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