Relation between induction of miR-15a, miR-16, miR-29b,and decrease of Mcl-1 and Bcl-2 in response to 10nM LBH589 in CLL cells. (A) Levels of Mcl-1, Bcl-2, GAPDH, and cleaved caspase-3 at 3, 6, 18, or 24 hours in 3 CLL samples that activated miR-15a, miR-16, or miR-29b in response to 10nM LBH589. (B) Quantitation of Mcl-1 levels at various times (up to 48 hours) in 9 CLL samples that activated miR-15a, miR-16, or miR-29b in response to 10nM LBH589. (C) Quantitation of Bcl-2 levels at various times (up to 48 hours) in 9 CLL samples that activated miR-15a, miR-16, or miR-29b in response to 10nM LBH589. (D) Levels of Mcl-1, Bcl-2, GAPDH, and cleaved caspase-3 at 3, 6, 18, or 24 hours in 3 CLL samples that did not activate miR-15a, miR-16, or miR-29b in response to 10nM LBH589. (E) Quantitation of Mcl-1 levels at various times (up to 48 hours) in 11 CLL samples that did not activate miR-15a, miR-16, or miR-29b in response to 10nM LBH589. (F) Quantitation of Bcl-2 levels at various times (up to 48 hours) in 11 CLL samples that did not activate miR-15a, miR-16, or miR-29b in response to 10nM LBH589.