HDAC expression in CLL and consequences of HDAC inhibition on chromatin marks at the miR-15a and miR-16 genes. (A) Expression of HDAC1-3 in CLL and normal lymphocytes. (B) Increased acetylation of histone H3 (H3K9/14) in CLL samples exposed to 10nM LBH589. (C-D) Increased levels of the H3K4me2 modification at the dleu2-miR-15a-16 promoter in CLL samples after exposure to 10nM LBH589 for 5 hours. (E) Induction of miR-15a and miR-16 in the same CLL samples after exposure to 10nM LBH589 for 5 hours.