B cells from patients with cGVHD have increased protein content and cell size. (A) B-cell protein content per million cells in peripheral B cells isolated from patients without active cGVHD (−cGVHD, n = 11) and with active cGVHD (+cGVHD, n = 9). Data are median ± range from 5 independent experiments. *P = .004 (unpaired 2-tailed t test). (B) B-cell size measured by forward scatter (FSC) in peripheral B cells from patients without cGVHD (−cGVHD) and with cGVHD (+cGVHD) after 24 hours of incubation. Left: Histograms of median FSC of a patient without active cGVHD (shaded area, thin line) and with active cGVHD (open area, bold line). Right: Median of FSC quantified in patients without cGVHD (−cGVHD, n = 5) and with cGVHD (+cGVHD, n = 3). Data are median ± range from 2 independent experiments. *P = .004 (unpaired 2-tailed t test).