SAXS analysis of the VEGF-E/VEGFR-2 ECD complex. (A) Scattering intensity and distance distribution function showing scattering data (blue dots), DAMMIF fit (red line), and SASREF fit (black dashed line). (B) Ab initio shape reconstruction of the VEGF-E/VEGFR-2 ECD complex. The mesh represents the average structure of 20 independent shape reconstructions. (C) Rigid-body homology modeling of the SAXS data to deduce the possible arrangement of the individual domains. VEGF-E is shown in blue; VEGFR-2 D23 is shown in dark gray; domains D1, D4, D5, and D6 modeled by c-KIT domains D1, D4, D5, and D5 (pdb 2E9W), respectively, are shown in pink; and the D7 dimer of VEGFR-2 is shown in purple (pdb 3KVQ). (D) Overlay of the independent ab initio and rigid-body homology models derived from SAXS data.