Thermodynamic analysis of VEGFR-2 D23 and full-length ECD binding to VEGF ligands. Top 2 rows show raw titration data of VEGFR-2 D23 and full-length ECD, respectively, with VEGF-A₁₂₁, VEGF-C, and VEGF-E. Bottom row shows integrated and concentration normalized isothermograms. Solid lines represent the best fit according to the “One Set of Sites” model.