Decorin is expressed in some putative components of hematopoiesis-supporting niches. Freshly isolated BM cells were sorted into the following populations: Lin⁻Sca-1⁺c-Kit^hi Flk-2⁻ HSCs; Lin⁻ Sca-1⁺ c-Kit^hi Flk-2⁺ Multipotent progenitor (MPPs); Lin⁻ Sca-1⁻ c-Kit⁺ myeloid progenitors (KL); Gr1⁺ CD115⁻ granulocytes (granulo); Gr1^hi/lowCD115⁺ monocytes (MO); Gr1⁻CD115l^ow/−F4/80⁺CD169⁺ macrophages (mac); CD45⁻Ter119⁻CD31⁺Sca-1⁺ endothelial cells (Endo); CD45⁻Ter119⁻CD31⁻CD51⁺Sca1⁺ MSCs; CD45⁻Ter119⁻CD31⁻CD51⁺Sca-1⁻ osteoblasts (OB); and CD45⁻Ter119⁻CD31⁻CD51⁺Sca-1⁻PDGFRβ⁺ CXCL12 abundant reticular cells. For experiment H, CD45⁺ hematopoietic cells (Hemato) and CD45⁻Ter119⁻CD31⁻ nonhematopoietic, nonendothelial cells (Stroma) were sorted from collagenase-treated spleens. Each set of panels represents results from RT-PCR normalized to samples with the smallest signals. When transcripts were detected in only one sample in an experiment, the results are shown as gel images.