Granzyme expression on (virus-specific) CD8⁺ T cells in the PB and LNs. (A) Representative dot plots of granzyme K and granzyme B staining on total (n = 11) and CMV-specific (n = 6, 2 donors were analyzed with 2 different tetramers), EBV-specific (n = 10, 3 donors were analyzed with 2 tetramers and 1 donor with 3 tetramers), and FLU-specific (n = 5) CD8⁺ T cells. (B-D) Comparison of percentages of granzyme-expressing cells within, from left to right: total, CMV-, EBV-, and FLU-specific CD8⁺ T cells between PB and LNs. (B) Granzyme B–containing cells. (C) Granzyme K–containing cells. (D) Granzyme B–containing and granzyme K–negative cells. Paired samples were analyzed using the Wilcoxon signed-rank test.