Expression of CD27 and CD45RA on (virus-specific) CD8⁺ T cells in the PB and LNs. (A) Representative dot plots of CD27 and CD45RA staining, gated on total (n = 15), CMV-specific (n = 10, 5 donors were analyzed with 2 different tetramers), EBV-specific (n = 7, 4 donors were analyzed with 2 tetramers and 2 donors with 3 tetramers), and FLU-specific (n = 5) CD8⁺ T cells analyzed in paired samples from the PB and LNs. (B) Comparison of percentages of CD27⁻CD45RA⁺ (effector-type) cells within total and CMV-, EBV-, and FLU-specific CD8⁺ T cells between PB and LNs. (C) Comparison of the percentages of CD27⁺CD45RA⁺ (naive) cells within total CD8⁺ T cells between PB and LNs. Paired samples were analyzed using the Wilcoxon signed-rank test.