Gata2 mRNA and protein levels are elevated in primary embryonic mouse LECs. (A) Quantification of Gata2 mRNA expression levels assessed by real-time RT-PCR in primary dermal LECs and BECs. Error bars represent mean ± SD of 3 replicates, and data are representative of 3 independent cell isolations from 20 to 30 embryos. (B) Primary dermal LECs and BECs cultured for 3 days on fibronectin and immunostained with CD31 (green), Prox1 (red), and Gata2 (cyan). Scale bar represents 30 μm. (C) Quantification of miR-451 expression levels assessed by real-time RT-PCR in primary dermal LECs and BECs. Error bars represent mean ± SD of 3 replicates, and data are representative of 3 independent cell isolations from 20 to 30 embryos. *P < .05 (Student paired t test).