Bcl2 expression is reduced in NHD13 LK cells. (A) Quantification of Bcl2 levels by qRT-PCR in LK cells from WT and NHD13 mice (n = 3 of each genotype, each sample is a pool of 3 mice) and marrow cells from NHD13 mice which developed AML (n = 5; **P < .01). (B) Representative histograms of Bcl2 protein in WT and NHD13 LK cells measured by FACS. The dashed lines represent the isotype controls. The mean cell fluorescence of Bcl2 protein in NHD13 LK cells relative to WT LK cells was calculated from 3-month-old mice (n = 3 of each genotype).