Distribution of TFPI isoforms for various endothelial cell lines. (A-B) EAhy926 cells. (C-D) SK–Hep-1 cells. (E-F) ECV304 cells. (G-H) HUVECs. (A,C,E,G) Washed cells were treated without or with PIPLC and analyzed for TFPI isoforms using immunoassays. PIPLC release was calculated by subtracting the without-PIPLC levels from the with-PIPLC levels. (B,D,F,H) Serum-free conditioned media were analyzed for TFPI isoforms using immunoassays.