Multilineage reconstitution of Balbc rag2^−/−γc^−/− mice with RDTR/SCFHA-LV–transduced hCD34⁺ cells. (A) FACS analysis of multilineage engraftment in BM of a Balbc rag2^−/−γc^−/− mouse reconstituted for 16 weeks with RDTR/SCFHA-LV–transduced CB CD34⁺ cells. The top right quadrants show the GFP⁺ cells within the early progenitors (hCD34⁺), B cells (hCD19⁺), myeloid progenitors (hCD13⁺), and monocytes (hCD14⁺) in the human graft (hCD45⁺ cells). FACS analysis of multilineage engraftment of the RDTR/SCFHA-LV–transduced CB CD34⁺ cells in the spleen and in PB is shown in panels B and C, respectively. Panel D shows the transduced cells in the thymic subpopulation: SP-4 (CD4⁺CD8⁻), SP-8 (CD4⁻CD8⁺), and DP (CD4⁺CD8⁺). The FACS profiles are representative of 6 experiments listed in Table 1.