DZNep inhibits histone methylation, reduces cellular Ezh2, and alters gene expression in activated CD8⁺ T cells. Naive CD8⁺ T cells were isolated from B6 mice and then stimulated with anti-CD3 and anti-CD28 Abs. Five days later, those activated T cells were further treated with or without DZNep. Cell lysates and RNA were prepared for measuring the amount of histone methylation, Ezh2 expression, and gene expression. (A) Western blot analysis shows the amount of histone methylation and acetylation in activated T cells treated with or without DZNep for 72 hours. (B) Graphs show (mean ± SD) the expression of genes associated with apoptosis and effector differentiation in activated T cells. (C) Western blot analyses show the expression of Bim protein in activated T cells treated with or without DZNep (200nM). (D) Western blot analysis shows the expression of Ezh2 in activated T cells treated with various doses of DZNep. (E) Graphs (mean ± SD) show the expression of genes repressed by Ezh2 in activated T cells treated with or without DZNep for 24 hours. Data are representative of ≥ 3 independent experiments.